Hello developer!
Now we have a problem. We want to extract the bubble sequence for subsequent annotation analysis. In the previous minigraph, we can use gfatools to extract for subsequent analysis, but the file generated by using pggb is empty.
We want to get the fasta file of non reference sequence and to find some genes are not in the reference genome.
Best everyday!
Hello developer! Now we have a problem. We want to extract the bubble sequence for subsequent annotation analysis. In the previous minigraph, we can use gfatools to extract for subsequent analysis, but the file generated by using pggb is empty. We want to get the fasta file of non reference sequence and to find some genes are not in the reference genome. Best everyday!
code: gfatools bubble graph.gfa >bubble.tsv