Closed brettChapman closed 1 year ago
Hi
I'm trying to run partition-before-pggb with latest 0.5.4 build on Docker.
I appears the build is pointing to the wrong location for scripts.
I get this error:
srun -n 1 singularity exec --bind /data/lupin_pangenome/pggb_run:/data/lupin_pangenome/pggb_run /data/pggb_builds/pggb_v0.5.4_Fatica.sif partition-before-pggb -i /data/lupin_pangenome/pggb_run/lupin_pangenome.fasta -s 5000 -l 0 -p 97 -n 7 -k 47 -B 10000000 -P asm20 -t 32 -T 16 -V L1:#:1000 -m -A -S -Z -o lupin_pangenome_s5000_l0_p97_k47_B10000000_G700-900-1100_Pasm20 [wfmash::map] Reference = [/data/lupin_pangenome/pggb_run/lupin_pangenome.fasta] [wfmash::map] Query = [/data/lupin_pangenome/pggb_run/lupin_pangenome.fasta] [wfmash::map] Kmer size = 19 [wfmash::map] Window size = 213 [wfmash::map] Segment length = 5000 (read split allowed) [wfmash::map] Block length min = 0 [wfmash::map] Chaining gap max = 50000 [wfmash::map] Percentage identity threshold = 97% [wfmash::map] Skip self mappings [wfmash::map] Mapping output file = /dev/stdout [wfmash::map] Filter mode = 1 (1 = map, 2 = one-to-one, 3 = none) [wfmash::map] Execution threads = 32 [wfmash::skch::Sketch::build] minimizers picked from reference = 62129839 [wfmash::skch::Sketch::index] unique minimizers = 3965697 [wfmash::skch::Sketch::computeFreqHist] Frequency histogram of minimizers = (1, 164401) ... (1315543, 1) [wfmash::skch::Sketch::computeFreqHist] With threshold 0.001%, ignore minimizers occurring >= 48030 times during lookup. [wfmash::map] time spent computing the reference index: 21.9314 sec [wfmash::skch::Map::mapQuery] mapped 100.00% @ 4.56e+06 bp/s elapsed: 00:00:24:07 remain: 00:00:00:00 [wfmash::skch::Map::mapQuery] count of mapped reads = 181, reads qualified for mapping = 182, total input reads = 182, total input bp = 6597089964 [wfmash::map] time spent mapping the query: 1.45e+03 sec [wfmash::map] mapping results saved in: /dev/stdout wfmash -s 5000 -l 0 -p 97 -n 6 -k 19 -H 0.001 -X -t 32 --tmp-base lupin_pangenome_s5000_l0_p97_k47_B10000000_G700-900-1100_Pasm20 /data/lupin_pangenome/pggb_run/lupin_pangenome.fasta --approx-map 40344.06s user 786.99s system 2798% cpu 1470.00s total 13161008Kb max memory python3: can't open file '/usr/local/bin/scripts/paf2net.py': [Errno 2] No such file or directory srun: error: node-10: task 0: Exited with exit code 2
Thanks.
Hi @brettChapman, I've just pushed a change that should fix your issue. Could you try again with the latest Docker build?
Thanks. I was able to run now to completion.
Hi
I'm trying to run partition-before-pggb with latest 0.5.4 build on Docker.
I appears the build is pointing to the wrong location for scripts.
I get this error:
Thanks.