Hi,
@pb-jlandolin
I download some test data from the ncbi, as the sra-tools do not provide a "pacbio-dump" like fastq-dump or illumina-dump, I used the commod "fastq-dump --table SEQUENCE *.sra" . But the sequence are all raw ZMW reads before filtering low quality and cutting adapters. My questions:
Is there a tool like "pacbio-dump" to extract the raw data (bax.h5) from sra data?
Or is there a tool to distinguish the pacbio adapters in raw ZMW reads ?
There is no pacbio-dump script. What you need to do is click on the SRR id, then click on the "Download" tab, and download the original bax.h5 files instead of the .sra files:
Hi, @pb-jlandolin I download some test data from the ncbi, as the sra-tools do not provide a "pacbio-dump" like fastq-dump or illumina-dump, I used the commod "fastq-dump --table SEQUENCE *.sra" . But the sequence are all raw ZMW reads before filtering low quality and cutting adapters. My questions:
Best Ryan