Open avilella opened 9 months ago
The error message says:
ERROR: CDS check file '/home/penguin/snpEff/./data/Khanna_j4h_8_DMX8/cds.fa' not found.
ERROR: Protein check file '/home/penguin/snpEff/./data/Khanna_j4h_8_DMX8/protein.fa' not found.
ERROR: Database check failed.
This means that there was no reference CDS or Proteins FASTA files to check against. SnpEff will refuse to save any database without checking it.
Here is the link to the documentation: https://pcingola.github.io/SnpEff/snpeff/build_db/#step-3-checking-the-database
I followed the instructions on how to build a .gtf file, for a simple use case where we have a CDS without introns from the ATG-to-the-stop codon.
When I create it in this manner:
I get the following report from running the
build
command:Any ideas where the issue could be? Do the reference sequences need to have a minimal UTR?