I am trying to annotate some VCF files with some COSMIC data (latest realeve V91) but the INFO filed for the COSMIC vcf has "=" in it and snpsift throws this error:
java.lang.RuntimeException: No white-space, semi-colons, or equals-signs are permitted in INFO field values. Name:"AA" Value:"p.W263R,p.W344R,p.W235R,p.W343R,p.L211P,p.W218R,p.L116P,p.P288=,p.W281R"
I am on version 4.3T
SnpSift version 4.3t (build 2017-11-24 10:18), by Pablo Cingolani
Here is the header of the COSMIC vcf:
`ryan@ubuntu:~/NGS/$ head -n 20 CosmicCodingMuts.vcf
fileformat=VCFv4.1
source=COSMICv91
reference=GRCh38
fileDate=20200324
comment="Missing nucleotide details indicate ambiguity during curation process"
comment="REF and ALT sequences are both forward strand
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
CHROM POS ID REF ALT QUAL FILTER INFO
1 65797 COSV58737189 T C . . GENE=OR4F5_ENST00000641515;STRAND=+;LEGACY_ID=COSN23957695;SNP;CDS=c.9+224T>C;AA=p.?;HGVSC=ENST00000641515.2:c.9+224T>C;HGVSG=1:g.65797T>C;CNT=1`
Hi,
I am trying to annotate some VCF files with some COSMIC data (latest realeve V91) but the INFO filed for the COSMIC vcf has "=" in it and snpsift throws this error:
java.lang.RuntimeException: No white-space, semi-colons, or equals-signs are permitted in INFO field values. Name:"AA" Value:"p.W263R,p.W344R,p.W235R,p.W343R,p.L211P,p.W218R,p.L116P,p.P288=,p.W281R"
I am on version 4.3T
SnpSift version 4.3t (build 2017-11-24 10:18), by Pablo Cingolani
Here is the header of the COSMIC vcf:
`ryan@ubuntu:~/NGS/$ head -n 20 CosmicCodingMuts.vcf
fileformat=VCFv4.1
source=COSMICv91
reference=GRCh38
fileDate=20200324
comment="Missing nucleotide details indicate ambiguity during curation process"
comment="URL stub for ID field (use the whole COSV identifier)='https://cancer.sanger.ac.uk/cosmic/search?genome=38&q='"
comment="REF and ALT sequences are both forward strand
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
INFO=
CHROM POS ID REF ALT QUAL FILTER INFO
1 65797 COSV58737189 T C . . GENE=OR4F5_ENST00000641515;STRAND=+;LEGACY_ID=COSN23957695;SNP;CDS=c.9+224T>C;AA=p.?;HGVSC=ENST00000641515.2:c.9+224T>C;HGVSG=1:g.65797T>C;CNT=1`
Thanks for any help you can provide!
Ryan