i was just starting to design some CRISPR guides to target the 5 candidate SNPs upstream of accA when i noticed something odd. all 5 are in a region of a single hexamer (GGGTTT) repeated 8 times in a row (see pdf). and all 5 of the SNPs occur in the same position of the hexamer (the 3rd G). this looks like it's more likely to be caused by an indel or structural variant rather than 5 different SNPs-- could you check the reads to see if this might be the case?
From Hunter: