The current 2022-08-17_ITS1_Oomycota_obs.fasta file was built against v0.12.5 and included in v0.12.6 onwards.
Right now with v0.12.9 repeating the procedure (with the DB including those sequences) results in shorter unknowns.fasta and empty file of observed left-extensions to be included.
Essentially we need to either make a temp DB without the old yyyy-mm-dd_ITS1_Oomycota_obs.fasta entries, or update yyyy-mm-dd_ITS1_Oomycota_obs.fasta incrementally.
Note that yyyy-mm-dd_ITS1_Oomycota_obs.fasta can lose entries as things are added to the w32 or curated files, as well as gain entries as we sequence more samples.
The current
2022-08-17_ITS1_Oomycota_obs.fasta
file was built against v0.12.5 and included in v0.12.6 onwards.Right now with v0.12.9 repeating the procedure (with the DB including those sequences) results in shorter
unknowns.fasta
and empty file of observed left-extensions to be included.Essentially we need to either make a temp DB without the old
yyyy-mm-dd_ITS1_Oomycota_obs.fasta
entries, or updateyyyy-mm-dd_ITS1_Oomycota_obs.fasta
incrementally.Note that
yyyy-mm-dd_ITS1_Oomycota_obs.fasta
can lose entries as things are added to the w32 or curated files, as well as gain entries as we sequence more samples.