petersaj / AP_histology

Histology processing
63 stars 20 forks source link

Identifying probe trace when it is across multiple slices #26

Closed cspurandare closed 12 months ago

cspurandare commented 1 year ago

Hi, I have just started using your histology pipeline and everything seems good and easy to use. I am encountering an issue where my probe did not end up in a single slice, but instead it traversed different depths in different (anterior to posterior) slices. The 8 slices are attached. I am at the step "AP_get_probe_histology", but 2 (4 files merged).pdf I am not sure if there is a way to assign probe track if it extends across multiple slices? As I understand it, the draw probe utility expects me to draw a line, but I dont know if it can stitch together different lines (or points) across different slices. Thanks and looking forward to your reply ! Chinmay

petersaj commented 1 year ago

Hi Chinmay - right that probe tracts almost never exist in just one slice: you mark the section of the probe visible on each slice by pressing the number corresponding to the probe (e.g. 1), then the final probe location is the line of best fit between all your marked points across all slices.

Apologies the documentation for this isn't great, I'm hoping to update it in the near future since I'm just getting back into histology processing