When identifying pbp5 PointFinder mutations using the included test data, the full set of 20 pbp5 mutations results in a BLAST percent identity of 98.233%. The default StarAMR BLAST percent identity threshold is 98%. After manually adding 2 codon mutations to the test file, the percent identity drops to 97.987% and no mutations (individual point or single complex) are reported.
Considering the mutations we're looking for in this region necessarily amount to a lower percent identity, I wonder if there is something we should do by default to accommodate the lower percent identity.
When identifying pbp5 PointFinder mutations using the included test data, the full set of 20 pbp5 mutations results in a BLAST percent identity of 98.233%. The default StarAMR BLAST percent identity threshold is 98%. After manually adding 2 codon mutations to the test file, the percent identity drops to 97.987% and no mutations (individual point or single complex) are reported.
Considering the mutations we're looking for in this region necessarily amount to a lower percent identity, I wonder if there is something we should do by default to accommodate the lower percent identity.