I make the variable names unique after read 10X H5 file to solve the errors and warnings when run merge_samples.py.
In brief, the error ValueError: cannot reindex from a duplicate axis raises from new_adata = all_data[0][2].concatenate(*[x[2] for x in all_data[1:]]); the warning Variable names are not unique from read_adata().
Below is the detailed message:
Variable names are not unique. To make them unique, call `.var_names_make_unique`.
reading: /WORKDIR/outs/filtered_feature_bc_matrix.h5 of type 10x_h5
(5659, 32285) /WORKDIR/outs/filtered_feature_bc_matrix.h5
/CONGA_DIR/conga/preprocess.py:210: DeprecationWarning: Use is_view instead of isview, isview will be removed in the future.
if adata.isview: # this is so weird
Variable names are not unique. To make them unique, call `.var_names_make_unique`.
reading: /WORKDIR/outs/filtered_feature_bc_matrix.h5 of type 10x_h5
(4861, 32285) /WORKDIR/outs/filtered_feature_bc_matrix.h5
/CONGA_DIR/conga/preprocess.py:210: DeprecationWarning: Use is_view instead of isview, isview will be removed in the future.
if adata.isview: # this is so weird
Traceback (most recent call last):
File "/CONGA_DIR/scripts/merge_samples.py", line 79, in <module>
new_adata = all_data[0][2].concatenate(*[x[2] for x in all_data[1:]])
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/anndata/_core/anndata.py", line 1705, in concatenate
pairwise=False,
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/anndata/_core/merge.py", line 815, in concat
[getattr(a, alt_dim) for a in adatas], alt_indices, merge
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/anndata/_core/merge.py", line 526, in merge_dataframes
dfs = [df.reindex(index=new_index) for df in dfs]
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/anndata/_core/merge.py", line 526, in <listcomp>
dfs = [df.reindex(index=new_index) for df in dfs]
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/util/_decorators.py", line 309, in wrapper
return func(*args, **kwargs)
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/core/frame.py", line 4036, in reindex
return super().reindex(**kwargs)
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/core/generic.py", line 4464, in reindex
axes, level, limit, tolerance, method, fill_value, copy
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/core/frame.py", line 3883, in _reindex_axes
index, method, copy, level, fill_value, limit, tolerance
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/core/frame.py", line 3905, in _reindex_index
allow_dups=False,
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/core/generic.py", line 4532, in _reindex_with_indexers
copy=copy,
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/core/internals/managers.py", line 1285, in reindex_indexer
self.axes[axis]._can_reindex(indexer)
File "/home/USER/.conda/envs/conga/lib/python3.6/site-packages/pandas/core/indexes/base.py", line 3292, in _can_reindex
raise ValueError("cannot reindex from a duplicate axis")
ValueError: cannot reindex from a duplicate axis
Hi there,
I make the variable names unique after read 10X H5 file to solve the errors and warnings when run
merge_samples.py
.In brief, the error
ValueError: cannot reindex from a duplicate axis
raises fromnew_adata = all_data[0][2].concatenate(*[x[2] for x in all_data[1:]])
; the warningVariable names are not unique
fromread_adata()
.Below is the detailed message: