While in a lot of regions P. pyralis has low variation, I've definitely run into some situations where the gene I've cloned has coding differences from the reference, and it is unclear to me if these coding differences are natural variants or PCR/cloning induced mutations.
Having a catalog of all the natural variation in our numerous RNA-Seq & Genomic seq datasets would be a useful contribution to the OGS.
While in a lot of regions P. pyralis has low variation, I've definitely run into some situations where the gene I've cloned has coding differences from the reference, and it is unclear to me if these coding differences are natural variants or PCR/cloning induced mutations.
Having a catalog of all the natural variation in our numerous RNA-Seq & Genomic seq datasets would be a useful contribution to the OGS.