search

pinellolab / CRISPResso2

Analysis of deep sequencing data for rapid and intuitive interpretation of genome editing experiments
Other
278 stars 95 forks source link

CRISPResso2 cannot handle drop out deletions between two target sites, apparently #387

Open GreenSeaBug opened 8 months ago

GreenSeaBug commented 8 months ago

Hello,

I am finding that reads containing a drop out deletion between two target sites do not map to my reference, and nor are they classified as ambiguous (reads without the drop out deletion do map to my reference, as expected). Is there a way to fix this?

Thank you.

GreenSeaBug commented 8 months ago

Apologies, solved with amplicon_min_alignment_score 0