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pinellolab / PrimeDesign

Software tool for the flexible design of pegRNAs and ngRNAs for prime editing!
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CFD Scores aren't in "Download Designs" spreadsheet #2

Closed marcus-r-kelly closed 3 years ago

marcus-r-kelly commented 3 years ago

I have been using the hosted PrimeDesign at https://drugthatgene.pinellolab.partners.org/. I have checked the option to calcuate CFD score, and these scores do display correctly in my browser for the sgRNAs. However, when I download designs using the "Download Designs" button, there is a column for CFD score in the spreadsheet for which no values are given. I suspect this is a bug.

jyhsu15 commented 3 years ago

Thank you for catching this, should be fixed now!

marcus-r-kelly commented 3 years ago

Thanks! Also, I notice that the CFD scores for all nicking guides in PE3b solutions have 0 CFD. I will admit that my understanding of CFD is not that deep, but is this correct?

jyhsu15 commented 3 years ago

Seems like calculating CFD is case-sensitive. Should be a super quick fix! Thank you.

marcus-r-kelly commented 3 years ago

Looks good now, thank you!