Hi! I am trying to generate a pegRNA to use for twin prime editing, i.e. inserting the full AttB site at a genomic site of interest, as discussed in this paper. Yet, AttB site is at least 38 bp long, while any insertion longer than 33 bp yields N/A as a suggested pegRNA. Is there an easy fix that doesn't involve re-training the model with longer inserts?
Hi! I am trying to generate a pegRNA to use for twin prime editing, i.e. inserting the full AttB site at a genomic site of interest, as discussed in this paper. Yet, AttB site is at least 38 bp long, while any insertion longer than 33 bp yields N/A as a suggested pegRNA. Is there an easy fix that doesn't involve re-training the model with longer inserts?