pombase / canto

The PomBase community curation tool
https://curation.pombase.org
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cant add CDT modified residue in Canto #2809

Closed ValWood closed 2 months ago

ValWood commented 2 months ago

I wanted to make these more specific rpb1 modifications more specific so that they would group correctly in the protein viewer

Screenshot 2024-03-05 at 16 37 28

oddly, they weren't in the session (even as existing), so they must come from the reciprocal of the CTD kinase S2 and S5 kinase annotations which is super impressive!

anyway the curation interface doesn't accept CTD_S2/5, I was able to work around using the "text" editor. This isn't a priority, as it only applies to rpb1 and I can do them by hand, but in case it is easy...

ValWood commented 2 months ago

Although oddly these don't get instantiated as modifications:

Screenshot 2024-03-05 at 16 48 51
ValWood commented 2 months ago

note to self, tomorrow these should be merged:

Screenshot 2024-03-05 at 16 53 50 Screenshot 2024-03-05 at 16 53 39
kimrutherford commented 2 months ago

Looks good!

image

ValWood commented 2 months ago

even better!

ValWood commented 2 months ago

Do you know why there are still 2 phosphorylation sites per position? I looked on the web page, and I cant see which annotations are causing this?

kimrutherford commented 2 months ago

Aren't these the annotations?

image

image

ValWood commented 2 months ago

I thought I fixed the, and I see this:

Screenshot 2024-03-09 at 06 53 06
kimrutherford commented 2 months ago

There are some phosphorylated residue annotations are only visible in the detailed view.

ValWood commented 2 months ago

OK! I thought I checked that. I will fix these on Monday

ValWood commented 2 months ago

In the curation session they look like this:

Screenshot 2024-03-09 at 07 27 18

will check on Monday

kimrutherford commented 2 months ago

Confusing!

I found them in pombe-embl/supporting_files/legacy_modifications_from_contigs.tsv after a bit of looking:

SPBC28F2.12     rpb1    MOD:00696       Inferred from Direct Assay      CTD_S2  added_by(PomBase:SPBC32H8.10)  PMID:19328067   4896    2009-07-12
SPBC28F2.12     rpb1    MOD:00696       Inferred from Direct Assay      CTD_S5  added_by(PomBase:SPBC19F8.07)  PMID:19328067   4896    2009-07-12
SPBC28F2.12     rpb1    MOD:00696       Inferred from Direct Assay      CTD_S2  added_by(PomBase:SPAC2F3.15)   PMID:19328067   4896    2009-07-12
SPBC28F2.12     rpb1    MOD:00696       Inferred from Direct Assay      CTD_S5  added_by(PomBase:SPBC32H8.10)  PMID:19328067   4896    2009-07-12
ValWood commented 2 months ago

fixed , with some others