chromosome segregation

[ValWood]

klp5, klp6 do not yet have phenotypes

• [x] increased chromosome loss ? (has a chromosome segregation phenotype)
• [x] elongated mitotic spindle ?

Csi1

• [x] is a kinetochore-SPB connector in mitotic interphase (Hou et al., 2012), paper is curated, mitotic centromere-SPB clustering protein, binds to sad1. More data required

Nuf2

• [x] which connects the centromeres to the SPB, disappears at the early meiotic prophase and releases the centromeres from the SPB (Asakawa et al., 2005; Hayashi et al., 2006.

need MF to capture this... attachment of mitotic spindle microtubules to kinetochore

Cut2

• [x] has no mitotic spindle annotation on dev, only meiotic (might have been made since).
  • [ ] Current annotation says cut2 is present during S-phase, check

Cut7

• [x] In the temperature-sensitive cut7 mutant, spindle formation is blocked shortly after duplication of the SPB. see https://github.com/pombase/fypo/issues/3415

• [x] The mitotic spindle radiating from the opposite poles cannot interdigitate and thus remains as a V shape (Hagan and Yanagida, 1992). see https://github.com/pombase/fypo/issues/3415

moved to https://github.com/pombase/curation/issues/2320

NMS complex

• [x] needs annotation to ndc80 complex

[ValWood]

sfi1

• [x] S. pombe Sfi1 (SpSfi1) perfectly colocalizes with Cdc31 at the SPB half-bridge/bridge in high-resolution fluorescence microscopy, and both proteins are essential for SPB duplication (48–50).

mitotic spindle pole body duplication   | cdc31, kms2, nda3, sfi1

mitotic spindle pole body duplication   | cdc31, kms2, nda3, sfi1

phew

bub1

• [x] protein localization to kinetochore-> histone H2A-S121 phosphorylation involved in protein localization t o centromere?

spc7

• [x] Is this turning SAC signalling off? (probably a phenotype bu OK for now)

[ValWood]

• [x] mis6, mis12 Fission yeast mis6-302 and mis12-537 mutants exhibit unequal segregation of regular chromosomes when shifted to a restrictive temperature. In these mutants, sister chromatids were segregated to the daughter cells at anaphase without a long delay, but the segregation patterns of sister chromatids were random. Consequently, these mutations produced aneuploid cells with a high frequency.

[ValWood]

• [x] reannotate ndc80 and MIND subcomplexes

  • annotate mis14 toMIND and move mis13 down to 'nuclear' moved to https://github.com/pombase/curation/issues/2321

• [ ] no substrates currently annotated for APC, look for motifs ABBA, KENm D-Box, C-BOX, F-box, 2BR, LRRL possible substrates to annotate mitotic securin and cyclin; late mitosis cdh1 , cytokinesis aurora, cyclins, cdc29; G1 geminin, cdc6, skp2,ubcH10(ubc11)....check all know APC targets annotated see https://github.com/pombase/curation/issues/2324

[ValWood]

• [x] dnt1 should this be in spindle checkpoint?

[ValWood]

• [x] Cells lacking either Klp5 or Klp6 are resistant to thiabendazole, a microtubule depolymerising agent,
• [x] display a prolonged delay in pro-metaphase and metaphase with highly elongated spindles and are
• [x] essential for viability in the absence of either Bub3 ...
• [x] or the Dam1 complex (Figure 3A, Garcia et al., 2002; Sanchez-Perez et al., 2005; West et al., 2002).

[ValWood]

Might be OK, the transition M/A was delayed . I wanted to check which 'genes' in metaphase have an "elongated spindle" phenotype, follow on from https://github.com/pombase/fypo/issues/2845

• [x] note added 1/02/2019 see https://github.com/pombase/curation/issues/2318

[ValWood]

• [x] find physical interaction to connect proteasome lid particle to core complex (currently the lid is floating around on its own in interaction network) see https://github.com/pombase/curation/issues/2319

[ValWood]

• [x] check all the cohesin/condensin ring have the GO:0061776 - DNA ring activity/topological entrapment term (also not dsDNA binding parent) https://github.com/pombase/curation/issues

[ValWood]

• [x] cut vs mitotic spindle assembly checkpoint override

[ValWood]

• [x] One key factor known to promote CENP-A loading is the conserved histone chaperone, Scm3/ HJURP, which is in turn recruited by the Mis16-Mis18 complex (Dunleavy et al., 2009; Foltz et al., 2009; Pidoux et al., 2009; Wil- liams et al., 2009; Yu et al., 2015). However, how CENP-A and histone H3 levels are precisely maintained at the centromere re- mains unknown.

[ValWood]

• [x] monopolar spindle check

In the first mutant phenotype category, the spindle formed from only one SPB, which is normally referred to as monopolar spindle (Figures 2A and S2A). This phenotype is common in a number of mutants for SPB components including Cdc31 [34], Sad1 [35], Cut12 [36], Cut11 [37], Pcp1 [38], Plo1 [39], and Cut7/Kinesin-5 [40].

I only have cut11, plo1 and cut7 from this list at present...

moved to https://github.com/pombase/curation/issues/2322

[ValWood]

• [x] skp1, bent spindle A similar phenotype has been reported for the skp1 mutant of the SCF (skp, cullin, F-box) ubiquitin ligase complex [30,43]. This is probably due to entangled chromosomes that fail to resolve during mitosis and meiosis, which is caused abnormal tension in the elongating spindle [30,43].

(this is 'curved spindle')

[ValWood]

In fission yeast, the recruitment of cohesin at the mating type locus, at outer repeat regions of centromeres, and at telomeric sites is dependent on the fission yeast heterochromatin protein 1 (HP1) homologue Swi6 through an interaction with Psc3 (Bernard et al., 2001; Nonaka et al., 2002) and the loading factor Mis4 (Fischer et al., 2009).

[ValWood]

• [x] Inhibition of Aurora B using a Shokat mutant leads to chromosome segregation defects (Koch et al., 2011), such as merotelic or syntelic attachment (Gay et al., 2012),

[ValWood]

gaaah this ticket is sooooo long ;(