ValWood
commented
5 years ago Translational Control of Cell Division by Elongator https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388810/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388810/figure/F2/
The Targets of Elongator Are Enriched in AAA and Fall into Four Groups of Functionally Related Genes Considering the specific defects resulting from the absence of the mcm5s2 modification, we postulated that mRNA-specific rather than transcriptome-wide translation defects would account for the observed phenotypes. To test this hypothesis, we combined the fission yeast integrated ORFeome strain library with Reverse Protein Macro-arrays (Matsuyama et al., 2006) to determine the relative expression of all fission yeast proteins in strains lacking either ctu1 or elp3. Expression levels were determined by normalization against a-tubulin whose level is not affected by the double modification (Figure S3). The 4910 tagged ORFs are expressed from the same promoter, terminator and chromatin locus in the various genetic backgrounds. Cluster analysis revealed an overall similarity in the protein expression profiles in the absence of either ctu1 or elp3 (Figures 3A and 3B), with a moderate global effect on the proteome: the average expression level of the proteome is 96% in the absence of ctu1 and 87% in the absence of elp3 (Table S2). In the Δelp3 strain, where the double modification is abolished, the expression level of 494 proteins was decreased (fold change > = 2). A gene ontology analysis revealed a significant enrichment of four distinct functional groups encompassing the regulation of the cell cycle, cytokinesis, response to nutrients, and chromatin modifications at the centromere (Figure 3B, Table S2). Further analyses confirmed that the protein expression level within these groups was statistically more affected than the whole proteome in the absence of elp3 (Figure 3C).
jrr-cpt
Analysis where GO is used substantially to support hypothesis driven questions.