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PomBase curation
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cdc2Hs . Canto complementation test #2382

Open Antonialock opened 5 years ago

Antonialock commented 5 years ago

there is an allele from pmid 8413179 called cdc2Hs(human cdc2 gene)

this sounds like a complementation experiment, do you remember Midori? You approved

mah11 commented 5 years ago

ask Jacky - it's one of her sessions @JackyVH

Also, this might fit in with some of the work @kimrutherford is doing for FlyBase - we could be getting close to having a way to do these in Canto because they need it.

JackyVH commented 5 years ago

Hi Both yes there is a cdc2Hs allele . The pombe cdc2 gene was replaced with the cdc2 human gene which substituted for the pombe gene and allowed cells to grow and divide . Hope this helps

Jacky Sent from my iPad

On 3 May 2019, at 13:01, Antonia notifications@github.com<mailto:notifications@github.com> wrote:

there is an allele from pmid 8413179 called cdc2Hs(human cdc2 gene)

this sounds like a complementation experiment, do you remember Midori? You approved

Antonialock commented 5 years ago

Maybe we should discuss how to do these...I’ve always just done a complementation annotation

ValWood commented 5 years ago

WE can move this to complementation once we can capture the specific phenotypes complemented

ValWood commented 5 years ago

waiting for https://github.com/pombase/canto/issues/826

Antonialock commented 5 years ago

By "waiting for pombase/canto#826 "

do you mean that you would like all our complementation data migrated to actual phenotype annotations (as opposed to simple classification e.g. partially complements, complements, doesn't complement) ? (if so, I wasn't aware)

ValWood commented 5 years ago

Yes longer term we would like to be able to specifiy which phenotypes are complemented. We discussed it at some point, possibly with flybase. It isn't a big deal for us as we don't have much complementation data that is really important. This one is somewhat a special case because these were the detailed asssays to showed that human and pombe cdk1s were functionally equivalent. I think we should record this for historical purposes ;)

kimrutherford commented 5 years ago

waiting for pombase/canto#826

826 has been closed so now: pombase/canto#1915

mah11 commented 4 years ago

tangent, but one more: S.p. dpm3 complements CHO Lec15 (cells don't express CHO dpm2; assay for transfected human CD59 product reaching cell surface - means GPI anchor restored) PMID:10835346

mah11 commented 4 years ago

another one: cdc42-G12V suppresses localization of scd1 phenotype of gef1delta PMID:31719163 (left comment on annotation in canto session 721bf4355105bfb4)

mah11 commented 4 years ago

PMID:9042863 34790a1dd257be4f cdc2-Y15F suppresses cell cycle arrest of chk1+ overexpression