I didn't do these (it's another 55!) would need to do all the singles, create the doubles and then do the phenotypes. This one can wait for the spreadsheet format...
The session is already quite unwieldy to find the allele you need.
Mis4/Scc2/NIPBL Suppressors May Restore Its Interaction with Psm1/3 Heads. To identify mutations that can compensate for mis4, we isolated a number of spontaneous suppressors for ts mutant mis4-G1326E (22), which had been integrated into the clean genome of wild-type fission yeast by homologous recombination. A number of Ts+ intragenic suppressors (listed in SI Appendix, Table S1C) were obtained within the mis4 gene, the majority of which were located in two narrow domains (amino acids 803– 844 and 1,332–1,387). Mis4/Scc2/NIPBL has the shape of a handle and a hook (16, 27).
ValWood
commented
5 years ago 
I didn't do these (it's another 55!) would need to do all the singles, create the doubles and then do the phenotypes. This one can wait for the spreadsheet format... The session is already quite unwieldy to find the allele you need.
Mis4/Scc2/NIPBL Suppressors May Restore Its Interaction with Psm1/3 Heads. To identify mutations that can compensate for mis4, we isolated a number of spontaneous suppressors for ts mutant mis4-G1326E (22), which had been integrated into the clean genome of wild-type fission yeast by homologous recombination. A number of Ts+ intragenic suppressors (listed in SI Appendix, Table S1C) were obtained within the mis4 gene, the majority of which were located in two narrow domains (amino acids 803– 844 and 1,332–1,387). Mis4/Scc2/NIPBL has the shape of a handle and a hook (16, 27).