MAPK pathway annotate atf4 substrates and inhibition of pmk1

[ValWood]

[ValWood]

Missing atf1 targets are also missing inhibition of pmk1 by pyp1/2 inhibition of pmk1 by ptc2/3

[Antonialock]

Fungal phosphorelays have three components (bacteria have two), i think this is how it happens:

1. Mak 1/2/3 are autophosphorylates on on a histidine, and then transfers the phosphate to an asp residue on itself
2. The phosphate is relayed to a histidine in the 'shuttle protein' Mpr1 (this protein is not present in bacteria), 3, the phosphate is relayed to an Asp residue in the response regulator Mcs4

it looks like the GO terms are specific to bacteria....maybe we need a fungal version of this pathway? "The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response."

[ValWood]

@Antonialock but what is the activity of mcs4?

[Antonialock]

I don't know, maybe it just causes a conformational change in bound proteins?

[ValWood]

protein kinase activator activity ! (SGD used)

[ValWood]

mcs4 targets are done. Still need to do:

• [ ] atf1 targets
  • [ ] inhibition of pmk1 by pyp1/2
  • [ ] inhibition of pmk1 by ptc2/3
  • [x] probably PMID:19625445 curated but not got that above annotations

[ValWood]

or

• [ ] PMID:17761528

pending https://github.com/PROconsortium/PRoteinOntology/issues/209

• [ ] We have recently shown that Pyp1, Pyp2, and Ptc1, whose transcriptional activation is dependent on Sty1– Atf1 function, interact also with Pmk1 in vivo and may participate in its dephosphorylation, thus providing a first evidence for the existence of “cross-talk” between the two MAP kinase (MAPK) pathways in S. pombe ......
• [ ] MAPK Sty1 is essential for proper deactivation of Pmk1 under hypertonic stress in a process regulated by transcription factor Atf1 through the action of tyrosine phosphatases Pyp1 and Pyp2, and serine/threonine phosphatase Ptc1
• [ ] We showed previously that osmo- stress-induced Pmk1 activation/deactivation in a strain ex- pressing a hyperactive version of Wis1 MAPKK (wis1DD), which prompts the constitutive activation of Sty1 (Shiozaki et al., 1998), is lower than in control cells due to increased synthesis of Pyp1, Pyp2, and Ptc1

[ValWood]

Hi @JackyVH PMID:19625445 reports pck2 as 'short'. I was going to classify as wee but it seems odd that this never came up in yours or Fran's screen. Do you think these cells are not small but stubby? To me here they look shorter, but n to really wider. What do you think? It's figure 1 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743619/

[ValWood]

• [ ] In fission yeast the stress-induced expression of pyp1 and pyp2 genes is regulated by Sty1 through Atf1 forming a negative-feedback loop

• [x] PMID:8649397 Degols et al., 1996; (curated) does not mention atf1

• [x] PMID:8824587 Shiozaki and Russell, 1996 (curated)

• [x] PMID:8824588 Wilkinson et al., 1996 (curated) decreased level of pyp2 transcript in atf1 mutants I can probably use

• [x] https://www.pombase.org/reference/PMID:17881729 this one is done

[ValWood]

Under moderate to high concentrations of hydrogen peroxide (naturally occurring, for example, in re- sponse to glucose arrest) nuclear accumulation of Pap1 is delayed until the expression of Atf1-dependent genes pro- motes its conversion to the active oxidized conformation that translocates into the nucleus and enhances Pap1-dependent gene expression (Madrid et al., 2004; Vivancos et al., 2006).

what is responsible for the activation of pap1?

[ValWood]

see https://github.com/pombase/curation/issues/2900