TORC1/2 signalling

[ValWood]

http://www.esyn.org/builder.php?projectid=1396&type=Graph

• [x] Add MFs from PMID:21035342 referred to in the review "Rab small GTPase emerges as a regulator of TOR complex 2"

• [x] Add Sat Starvation induced arrest add name derivation to all

Refine products:

• [x] sat1 SPBC23E6.08. Rab-specific(Ryh1/Ypt6) Riv1-Rgp1 guanyl-nucleotide exchange factor subunit Rgp1/Sat1. Add primary name rgp1
• [x] sat4 SPAC1851.04c Rab-specific(Ryh1/Ypt6) Riv1-Rgp1 guanyl-nucleotide exchange factor subunit Ric1/Sat4
• [x] sat7 SPAC4C5.02c. Rab family GTPase Ryh1

These phenotypes are still missing, all others mentioned are present sterility - defective response to nitrogen starvation

• [ ] abnormal cellular response to nitrogen starvation (FYPO:0000152)
• [ ] sterile (FYPO:0000280)

[ValWood]

[ValWood]

Tor should have mating without nitrogen starvation? Another important phenotype related to Tor2 activity is the inhibition of sexual differentiation. Inactivation of Tor2 in rich medium leads to cell cycle exit, G1 arrest, sexual differentiation, meiosis and sporulation (Alvarez and Moreno, 2006; Hayashi et al., 2007; Urano et al., 2007; Uritani et al., 2006).

[ValWood]

The subcellular localization of TORC2 is also a subject for ongoing research. Like in higher eukaryotes, S. pombe TORC2 has been local- ized to the plasma membrane, but it is also found in the nucleus (reviewed in [9]).

[ValWood]

ste20, rictor/ alreade curated

• [x] PMID: 19417002

• [x] PMID: 18076573

• [x] PMID: 15731009 (not TOR, other ste20)

• [x] PMID: 8943016 (not TOR, other ste20)

• [ ] PMID: 23271606

• [x] PMID: 10467002

• [x] PMID: 17261596 mating efficiency-> mating in the absence of blah

• [ ] tip41 connect? PMID:16297994

• Overexpression of tip41(+) increases the activity of type 2A phosphatase

• In a ppa2 deletion strain with reduced PP2A activity, overexpression of tip41(+) no longer blocks the shift to G1 upon nitrogen starvation. also mailed SGD for ortholog annotation

[ValWood]

Gsk3 is an upstream regulator affecting the TSC2 inhibitor of TORC1 (25), which is conserved in fission yeast (26) and has also been shown to function downstream of TORC1 (27).

25. Inoki, K. et al. TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth. Cell 126, 955–968, doi: 10.1016/j.cell.2006.06.055 (2006).
26. Matsumoto, S., Bandyopadhyay, A., Kwiatkowski, D. J., Maitra, U. & Matsumoto, T. Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe. Genetics. 161, 1053–1063. (2002).
27. Zhang, H. H., Lipovsky, A. I., Dibble, C. C., Sahin, M. & Manning, B. D. S6K1 regulates GSK3 under

[ValWood]

• [x] Previous studies have shown that AMPK negatively regulates TORC1-related processes28.

This is the paper I still have in approval

[ValWood]

This is through, but I still need to add some extensions. Also I thought that the ppk34->ssp2-> torc1 connections were direct but it seems there is stuff in between these so I wouldn't be able to complete this pathway yet.

[ValWood]

Specific entities upstream of TORC pathway (positive and negative regulators) should have +/- regulation of autophagy +/- regulation of translation and other anabolic processes (& growth) +/- regulation of cell cycle switching