Shugoshin /rec8/ protein phosphatase 2A (PP2A) cohesion protection pathway

[ValWood]

This connection is missing:

Shugoshin forms a complex with protein phosphatase 2A (PP2A) at the centromeres

• [x] PMID:16541025 Kitajima et al. 2006;
• [x] PMID:16541024 Riedel et al. 2006; up to Is PP2A-p sufficient to confer protection of Rec8? ~Tang et al. 2006~ human ~Lee et al. 2008~ human ~Llano et al. 2008~ mice

is sgo1 even sequestering?

and antagonizes Rec8 phosphorylation, a pre- requisite for cleavage by separase in meiosis I

• [x] Ishiguro et al. 2010; ~Katis et al. 2010~ yeast paper

[ValWood]

-[ ] https://www.pombase.org/reference/PMID:28497540 needs active forms, and appropriate phosph-recruitment adding adding

[ValWood]

Does Sgo1 sequester rec8 or just provide a binding platform for PP2a? PP2A then keeps rec8 dephosphorylated which protects it from cleavage?

"In budding yeast and human cells, phosphorylation of the Scc1 subunit by Plk1 enhances its cleavability by separase17,19,20, and this may similarly apply for the meiotic counterpart Rec8 (refs 21, 22). Therefore, a mechanism to protect cohesin at centromeres might be to inhibit its phosphorylation, but no evidence for this has been obtained as yet. It is also unknown whether shugoshin uses a similar mechanism to protect centromeric cohesin in both mitosis and meiosis. Therefore, we have investigated the mechanism by which shugoshin protects cohesin at the centromere. "

[ValWood]

Polo kinase phosphorylates cohesion (which subunits/residues?) Plo1-> scc3 (not sure this is yet available for fission yeast, possibly only shown in human/cerevisiae)

[ValWood]

Check modifications for cohesion protection pathway

[ValWood]

out of date http://noctua.geneontology.org/workbench/noctua-alliance-pathway-preview/?model_id=gomodel%3A66187e4700001744 there outstanding tasks in the spreadsheet