questions related to mitotic catastrophe phenotypes

[ValWood]

1. Loss of the Wee1 and Mik1 kinases, which catalyze Y15 phosphorylation, leads to mitotic catastrophe, in which cells enter mitosis at an inviably small size (Lundgren et al. 1991).

2. Cig2/CDK is required for mitotic catastrophe in the absence of Cdc2 Y15 phosphorylation (Bueno and Russell 1993; Zarzov et al. 2002).

(Furthermore, the requirement of Cig2 for mitotic catastrophe in the absence of Cdc2 Y15 phosphorylation explains the viability of Cdc13-Cdc2 single-chain CDK cells lacking Y15 phosphorylation without the need for further mechanisms of CDK regulation.)

however we have

FYPO:0003165 | cut with abnormal chromosome segregation has penetrance low | Microscopy | + Calcofluor White temperature shift, standard to high + HU

for cig2Δ mik1Δ wee1-50(G850E aa) which is essentially a 'mitotic catastrophe' without cig2 and cdc Y15 phosphorylation....

Is that correct?

@JackyVH

[ValWood]

Figure 1 cig2D suppresses the mitotic catastrophe phenotype of wee1-50ts mik1D cells leads to mitotic catastrophe, in which cells enter mitosis at an inviably small size (Lundgren et al. 1991).