ValWood
commented
2 years ago Annotation comes from https://www.pombase.org/reference/PMID:9872416 but this is inappro[private because they didn't isolate the genes. For the ones for which there is only one candidate, I will keep but change to TAS (traceable author statement). I will remove all annotation to SPAC3C7.13c from this paper.
While the functions of two previously predicted glucose-6-phosphate 1-dehydrogenases have been more confidently annotated (see Corkins et al., JCB 2017, which is linked in Pombase):
...one of these three proteins was left uncharacterized by this work:
According to sequence trait, domain annotations (reflected in Pombase), SPAC3C7.13c is absent the glucose-6-phosphate 1-dehydrogenase active site.
PaperBLAST of the SPAC3C7.13c sequence did not provide any further characterizations specific to this protein; however, it should be noted that all three of these proteins are comprised of a C-terminal Dihydrodipicolinate Reductase domain while, at the same time, it does not appear that this enzyme or activity has been identified in Pombase.
Searches of "Dihydrodipicolinate Reductase", "dapB", "4-hydroxy-tetrahydrodipicolinate reductase" or "2,3,4,5-tetrahydrodipicolinate:NAD(P)+ oxidoreductase." in Pombase and YGDb return 0 useful/true-positive hits. Literature searches of these terms with reference to "pombe" were also unproductive. Clarification of GO terms surrounding this biochemistry may be necessary (?).
Has this enzyme or respective pathway been identified in S. pombe?