ValWood
commented
2 years ago I think these can probably mostly be promoter:gene 'wild type' [expression level]
res1+OE,SPBC725.16:allele-35,nmt1-res+,{4fc60b01472378b1} nmt41-tap42,SPCC63.05:allele-2,thiamine-repressible,{5bef3e6a63b5bcf4} nmt1-res2,SPAC22F3.09c:allele-7,nmt1-res2+,{2467c1608e001a46} cdc13+(nmt81-SO),SPBC582.03:allele-15,switch-off,{c396e950a0cdf1ad} nmt1-ctr4-YFP,SPCC1393.10:allele-2,ORFeome construct,{7792eb73b01ab912} nmt81-pcp1,SPAC6G9.06c:allele-2,nmt81-pcp1+,{1ebe4a1548de4aff} cdt1+(nmt81-SO),SPBC428.18:allele-4,switch-off,{c396e950a0cdf1ad} nmt81-vid21,SPCC1795.08c:allele-3,depletion,{7927a4b16214fe51} nmt-cdc13+,SPBC582.03:allele-13,cdc13 switch off,{2467c1608e001a46} nmt81myc cdc18+,SPBC14C8.07c:allele-21,myc1 cdc18,{5ef3337d730491a3} cig2+ OE,SPBC11B10.09:allele-77,nmt1-cig2+,{4fc60b01472378b1} res1+HA OE,SPBC725.16:allele-32,nmt81-res1+ HA,{4fc60b01472378b1} nmt1-seb1,SPAC222.09:allele-3,Depletion with thiamine,{216b45645076768d,4fb0ad7bc2d593e0} P81nmt1-rrp41,SPAC3G9.10c:allele-3,Depletion with thiamine,{f2202f3e99350d80} wee1-OP,SPCC18B5.03:allele-12,pnmt1-wee1+,{9a3197e7865f3d22} cdc18+(nmt81-SO),SPBC14C8.07c:allele-51,switch-off,{c396e950a0cdf1ad} nmt41 cdc13,SPBC582.03:allele-19,switch off,{370824a189ad6763} nmt41-cdc10+,SPBC336.12c:allele-16,nmt41-cdc10+,{2467c1608e001a46} nmt1-tea1+,SPCC1223.06:allele-14,nmt1-tea1+,{f6b6af4b58d157ce} nmt81-sec8,SPCC970.09:allele-3,shut-off allele,{ce86e0783b08ae9a,50905b15a05554bd} P81nmt1-dis3,SPBC26H8.10:allele-4,Depletion with thiamine,{f2202f3e99350d80} nmt81cdc18+,SPBC14C8.07c:allele-22,cdc18+,{5ef3337d730491a3} nmt1-Ccp1,SPBC36B7.08c:allele-4,nmt1-Ccp1,{c2a8d0f860ace791} nmt1:skb15+,SPCC16C4.08c:allele-4,nmt1-skb15+,{b3bc9cbfdcacc24c} P81nmt1-mtr4,SPAC6F12.16c:allele-2,Depletion with thiamine,{f2202f3e99350d80} nmt1-pcf11,SPAC4G9.04c:allele-3,Depletion with thiamine,{216b45645076768d} nmt1-res1+,SPBC725.16:allele-11,nmt1-res1+,{2467c1608e001a46,4fc60b01472378b1}
manulera
1- Most should be describable as nucleotide mutations (not urgent)
2- Some can be moved to "promoter swap" if we create a type for that? but should we consider the purpose of the swap (i.e to overexpress or knockdown?)
3.I have been recording some promoter constructs like this
i.e wt with a prefix promoter: (it seems to be OK to edit the default gene label for WT), but I don't know if creating different wt constructs for the same genes will cause a namespace clash. I need to check this.
sms1-23,SPBC1198.12:allele-2,promoter mutant,{9d547e188c364e28} ntp1-CRE-like promoter mutant,SPBC660.07:allele-2,TGACGTAG mutated to TGACcTAG,{70548d974e6b7ebf,54a26a25997d999a} mad1=500%,SPBC3D6.04c:allele-31,nmt1 promoter,{ed9652a888c24c94} mad1=10%,SPBC3D6.04c:allele-29,nmt81 promoter,{ed9652a888c24c94} cup1-TT,SPBC17G9.13c:allele-3,cup1-promoter-> ura4 transcription teerminator,{c2cd1d8a4d29ba93} slp1=40%,SPAC821.08c:allele-11,rad21 promoter,{f8b9e68a8b938c8d,ed9652a888c24c94} trz2(promoter--110--54,G-102C,T-99A),SPBC3D6.03c:allele-3,-110--54,G-102C,T-99A,{7777e3bab77e1283} ntp1-MVP15,SPBC660.07:allele-6,HSE2 promoter mutant TTCGTGAATAGGAA->TTTGTAAATAGAAA,{54a26a25997d999a} apc14-promoter sequence mutant,SPAC27D7.05c:allele-3,point mutation in FLEX-like promoter,{e930f865a30e2c82} fus1-TR1 and TR2 deletion,SPAC20G4.02c:allele-26,deletion of both TR boxes in promoter,{ef2da839886aa260} nmt41-CENP-A-Cnp1,SPBC1105.17:allele-4,nmt41 promoter,cnp1+ coding,moderate overexpression level,{c6ced0325cbf00af} mad1=300%,SPBC3D6.04c:allele-32,ark1 promoter,{ed9652a888c24c94} spo6DF,SPBC1778.04:allele-3,FLEX-D core heptamer deleted from promoter,{07dd8e6bdc30211a} mad2=10%,SPBC20F10.06:allele-15,mad3 promoter,{ed9652a888c24c94} nmt1-CENP-A-Cnp1,SPBC1105.17:allele-5,nmt81 promoter,cnp1+ coding,high overexpression,{c6ced0325cbf00af} cdc25-dF1234,SPAC24H6.05:allele-25,4 FLEX promoters deleted,{0cad091b0886ebbf} pnmt81-cdr2,SPAC57A10.02:allele-10,nmt81 promoter,cdr2+,{bbe966c7c31d9042} cut1OE,SPCC5E4.04:allele-31,nmt1 promoter,{ff759b3e7d7da717} srw1-MutPste9,SPAC144.13c:allele-7,mutated promoter region,{f372ba194a5d127c} cut8+OE,SPAC17C9.13c:allele-5,cut8 nmt1 promoter,{f8b9e68a8b938c8d} mad2=65%,SPBC20F10.06:allele-12,hphNT1 cassette in promoter,{ed9652a888c24c94} ntp1-MVP5,SPBC660.07:allele-3,605 bp promoter deletion,{54a26a25997d999a} ntp1-MVP7,SPBC660.07:allele-4,464 bp promoter deletion,{54a26a25997d999a} ppc89-nmt81,SPAC4H3.11c:allele-5,ppc89-nmt promoter,{feac1fe40f21f7a9} scd1-low,SPAC16E8.09:allele-3,repression using nmt81 promoter,{3d43cf9a14d68a43} prz1-promoter-mutant,SPAC4G8.13c:allele-3,delta-1.2--1.5kb,{9618d1df6e9897a0} mad1=30%,SPBC3D6.04c:allele-30,bub3 promoter,{ed9652a888c24c94} ssm4-prom::ura4,SPAC27D7.13c:allele-8,ura4 transcription terminator inserted at ssm4 promoter,{68637de9f01b5872} fus1-TR1 deleted,SPAC20G4.02c:allele-28,TR1 promoter box at -157 deleted,{ef2da839886aa260} Pnmt81-mzt1,SPAC9G1.15c:allele-6,nmt81 promoter shut-off,{7b2ad53e7a1727a2,7b2ad53e7a1727a2} p3nmt-rnh1,SPBC336.06c:allele-3,p3nmt Thiamine repressible promoter,{b2192f51379c99f3} ste1-promoter(deltaFLEX1),SPBC32C12.02:allele-24,flex1delta,{5fd244fcaaf1c2fa} nmt81-CENP-A-Cnp1,SPBC1105.17:allele-3,nmt81 promoter,cnp1+ coding,weak overexpression level,{c6ced0325cbf00af} ntp1-MVP14,SPBC660.07:allele-5,HSE1 promoter mutant GAAGGAAACGGAA->AAAGAAAACGAAA,{54a26a25997d999a} mad3=30%,SPCC1795.01c:allele-18,hphNT1 cassette in promoter,{ed9652a888c24c94} prad21-cdc25,SPAC24H6.05:allele-27,rad21 promoter control,{915dd27f11d0b65d}
(I changed the ~ to = so they didn't look x'd out)