ValWood
commented
8 years ago not much I can do with the others, paralogy etc making predictions difficult. Lack of non-generic GO terms for cytoplasmic ribosome assembly and can't specify a particular subunit. Will leave as is for now
hpm1 YIL110W SPAC1071.05(conserved unknown) (DONE) rmt1 YBR034C SPAC890.07c (has ribosome export) rmt2 YDR465C SPAC26A3.17c (has ribosome biogenesis) rkm1 YPL208W set8 (SPAC3C7.09) and set10 (SPBC1709.13c) rkm2 YDR198C set11 SPCC1223.04c has ribosome biogenesis rkm3 YBR030W rkm4 YDR257C set13 (SPAC688.14), SPBC16C6.01c conserved unknown rkm5 YLR137W ntm1 YBR261C SPAC16E8.14c (has translation) sfm1 YOR021C (NO ORTHOLOG, checked, family absent)
Look for missing orthologs Update products and ISS whether involved in biogenesis, elongation, termination in PMID: 26801560
This suggests that these methyltransferases (Hpm1, Rkm1, Rmt1, and Rmt2) are likely active participants during the assembly process of the ribosome. Ntm1 may not be actively involved in the assembly process of the ribosome but instead methylation of its ribosomal protein substrate, Rpl12, may be important for ribosomal assembly,
Rkm2 and Ntm1 are important in translation termination efficiency the rest are translation
Loss of Rkm2, Rkm3, Rkm4, Rkm5, or Sfm1 had little or no impact on the levels of ribosomal components, suggesting they are not required for ribosomal subunit synthesis, but all (except rkm2 and Ntm1) are involved in translation elongation fidelity.