various cdc2 annotation. phosphorylation. phenotype

[ValWood]

We have a lot of known info missing from here:

[ValWood]

1. we know that cdc25 removes Y15 this isn't captured
2. In fact, cdc2p is phosphorylated primarily on two residues in fission yeast, Y15 and T167 (Gould and Nurse 1989; Gould et al. 1991), although T14 phosphorylation can also be detected under certain circumstances so if Y15 is the only tyrosine phoshorylated, this must always be what was assayed here?

Can we fill this in for others?

1. some tyrosine 15 is reported as T15
2. phosphorylated residue Y15 should be tyrosine phosphorylation

[ValWood]

Tryptic cleavage of 32P-labeled Schizosaccharomyces pombep34cdc2generatedonlytwophosphopeptides,termed 1 and 2 (Gould and Nurse, 1989). Phosphopeptide 1 contained phosphorylated Tyrl5 and phosphopeptide 2 contained phosphothreonine (Gould and Nurse, 1989).

This is prior knowledge so we can assume that in subsequent papers they knew which residue they were assaying.

[ValWood]

upon shift to non-permissive temp cdc2-M63 arrests in G1 and G2 but not in mitosis (Nurse et al 1976)

[ValWood]

I have positive regulation of SIN, but this implies that it negatively regulates SIN, check

During mitosis in fission yeast, active cdc2/cyclin kinase prevents cytokinesis before anaphase by inhibiting actomyosin ring constriction and septation. This control mechanism prevents the constricting ring and the septum from cutting the nuclei in half before sister chromatid segregation (Balasubramanian et al., 2000; Le Goff et al., 1999).

Therefore, downregulation of cdc2/cyclin in anaphase is essential for cytokinesis (Cerutti and Simanis, 1999; He et al., 1997; Kim et al., 1998).

[ValWood]

find a paper for meiotic exit for cdc2

[ValWood]

at the end, check for consistency between cdc13 and cdc2 annotations

[ValWood]

Most of this is done. transferring bits to a new ticket.