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Fission Yeast Phenotype Ontology
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inviable after spore germination, without cell division, spheroid cell #1130

Closed fypoadmin closed 9 years ago

fypoadmin commented 11 years ago

PMID:9078365 / 91d681458c26c2f0

Original comment by: mah11

fypoadmin commented 11 years ago

or could just go straight for "spherical"

Original comment by: mah11

fypoadmin commented 11 years ago

How can you be "viable without cell division?

Original comment by: ValWood

fypoadmin commented 11 years ago

Original comment by: mah11

fypoadmin commented 11 years ago

erm, by the Awesome Power Of Typo :P

Original comment by: mah11

fypoadmin commented 11 years ago

(although if you look beyond yeast, there are all those terminally differentiated living cells in bigger eukaryotes ...)

Original comment by: mah11

fypoadmin commented 11 years ago

Ah right, my original post was going to be" I should check with Jacky, but I think that all "inviable after spore germination, without cell division" might be spheroid if they are not otherwise misshapen as they remain as a spore..... so i will check this and if so, this is a not to self to map all of the deletions with this phenotype to the more specific term.....

Original comment by: ValWood

fypoadmin commented 11 years ago

Actually, can you be "after spore germination" and still round? I thought we the start of germination was symmetry breaking?

Original comment by: ValWood

fypoadmin commented 11 years ago

All I know is that in PMID:9078365 the authors say their paa1delta spores have germinated -- I don't know how they can tell; I'm taking their word for it -- but the resulting cells stay rounded.

Original comment by: mah11

fypoadmin commented 11 years ago

Hmm, OK. I remember a conversation with Jacky that you could tell if a cell had germinated if it had "broken symmetry". I germination may happen earlier but she did not know of a visual clue. I guess these authors must have some way of determining this...it would be interesting to know what it is...no other clues?

I will recheck with Jacky...

VAl

Original comment by: ValWood

fypoadmin commented 11 years ago

It would seem odd though, if germination is "emerging from a spore" and they are still round?

Original comment by: ValWood

fypoadmin commented 11 years ago

well, nothing else that I recognized as a clue ... full text is here, if you want to see if you can figure it out:

http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2443.1996.02002.x/pdf

Original comment by: mah11

fypoadmin commented 11 years ago

Actually I see in GO it is :

The physiological and developmental changes that occur in a spore following release from dormancy up to the earliest signs of growth (e.g. emergence from a spore wall).

so that fits if emergence is the final stage. Presumably they have some other evidence for release from dormency?

Original comment by: ValWood

fypoadmin commented 11 years ago

I guess they must, although I don't think I caught what it is (if they said).

Original comment by: mah11

fypoadmin commented 11 years ago

I would just continue as you were. I will ask Jacky re the deletions as it isn't really clear cut if something is not sporulated normally, or germinated but still round.....sigh...

Original comment by: ValWood

fypoadmin commented 11 years ago

I can't see in the paper either what it is which indicates they are germinated. I asked Jacky as might be useful to know for future.

Val

Original comment by: ValWood

fypoadmin commented 11 years ago

Original comment by: mah11

fypoadmin commented 11 years ago

inviable after spore germination, without cell division, spheroid cell FYPO:0002762

Original comment by: mah11