ectopic expression alleles PMID:34851403

[ValWood]

e.g. What did we say we would do for these? moa1-7dsr(ntA63G,A96G,C99T,A183G,C186T,T222G,A225G,T285C,A288G, T294G,A297G,A324G,C327T)[Ectopic]

This allele is ectopically expressed in mitosis

was the plan to create an ectopic expression phenotype branch in FYPO?

[ValWood]

Here core cohesion is induced in mitosis, so maybe

centromere core cohesion in mitosis
and sister kinetochore fusion in mitosis is enough (this process only happens usually in meiosis I)

[ValWood]

Add promoter when avaiable

[ValWood]

These results suggest that Moa1 is functional in mitosis. In addition, the expression of rec8 and/or moa1-7dsr slightly or hardly reduced cell viability (Fig. S4E), demonstrating that these factors do not severely compromise chromosome segregation in mitosis. The expression of rec8 reduced separation of sister kinetochores and centromere cores at non-significant levels (Fig. 5D; Fig. S5A.B). By contrast, moa1-7dsr expression alone also reduced kinetochore and centromere core separation at significant levels (Fig. 5D; Fig. S5A.B). This result indicates that Moa1 is able to affect kinetochore and centromere organization in the absence of Rec8. Interestingly, the separation frequencies were reduced in cells simultaneously producing Rec8 and Moa1-7dsr to a similar level to that of Rec8 alone (Fig. 5D; Fig. S5A,B), suggesting a functional relationship between Rec8 and Moa1. Despite an alteration in separation of sister kinetochores and centromere cores, separationwas not eliminated (Fig. 5D), and the shape of non-separated signals was not significantly altered in Rec8- and/or Moa1-7dsr-producing cells (Fig. 5E; Fig. S5C,D). Therefore, although Rec8 and Moa1-7dsr have an impact on sister kinetochore organization, they are not sufficient for the induction of sister kinetochore association in mitosis.

I decided not to curate this for now because it would involve complicated phenotype terms that would not necessarily make sense in FYPO other than through the lens of ectopic localization, and could be more confusing than useful. This branch is already very complicated. I might revisit when GO terms and FYPO terms have been reviewed.