Issue with file format

[glabouebe]

Hello, when I try to load a .avi file I got this error:

Unable to perform assignment because the size of the left side is 256-by-256 and the size of the right side is 256-by-256-by-3.

Error in read_file (line 63) imData(:,:,i-sframe+1) = Y1;

Error in ez_roi_detect/RunROIDetectionButtonPushed (line 337) Y = read_file(filename);

Error using matlab.ui.control.internal.controller.ComponentController/executeUserCallback (line 386) Error while evaluating Button PrivateButtonPushedFcn.

I obtained the same error for both motion correction and ROI detection function. Could you please help me with that ?

Thank you a lot in advance for your help.

Best.

[bodayw]

Hi,

From your error message:

Unable to perform assignment because the size of the left side is 256-by-256 and the size of the right side is 256-by-256-by-3.

This suggests that your video file has a 256x256 resolution, and has 3 channels per pixel (likely RGB), i.e. there are 3 values for each pixel.

However, EZcalcium can only handle videos that contain single-channel images (aka grayscale images), i.e. each pixel can only has 1 value.

So you would need to prepare single-channel image data files for EZcalcium - either select the right format when "saving" or "exporting" your data files in whatever image acquisition/processing software, or "convert" your existing files to single-channel (grayscale) format.

[glabouebe]

Hello,

Thank you so much for your help. I'm gonna try what you suggested !

Best regards,

Gwen.

Le mar. 6 juil. 2021 à 12:52, bodayw @.***> a écrit :

Hi,

From your error message:

Unable to perform assignment because the size of the left side is 256-by-256 and the size of the right side is 256-by-256-by-3.

This suggests that your video file has a 256x256 resolution, and has 3 channels per pixel (likely RGB), i.e. there are 3 values for each pixel.

However, EZcalcium can only handle videos that contain single-channel images (aka grayscale images), i.e. each pixel can only has 1 value.

So you would need to prepare single-channel image data files for EZcalcium

• either select the right format when "saving" or "exporting" your data files in whatever image acquisition/processing software, or "convert" your existing files to single-channel (grayscale) format.

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