The results are very different in Panel "amplification-details" and "Test your primer set"

pr2database / pr2-primers

Scripts for paper Vaulot et al. 2022

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The results are very different in Panel "amplification-details" and "Test your primer set" #14

Open marineLZ opened 1 year ago

marineLZ commented 1 year ago

I test the Primer 1380F CCCTGCCHTTTGTACACAC -1510R CCTTCYGCAGGTTCACCTAC , in the test primer set module, only 26.88% sequences matching revervse primer. However, for the same primers in the Amplification-details, 79.17% could match reverse primer. Why ?

$I2S_733GN{RWQVTLBRH{GR2$ CV}%4T{HA DRXOJTWH~{`PK