original references for primers 329F/329R and 528F

[frederic-mahe]

Quick note. The publication Guillou et al. 2004 gives older references than the one we currently have for primers 329F/329R and 528F:

The entire SSU rDNA gene was amplified using the eukaryotic primers Euk 328f (5′-ACC TGG TTG ATC CTG CCA G-3′) and Euk 329r (5′-TGA TCC TTC YGC AGG TTC AC-3′) as described in Moon-van der Staay et al. (2001).

Partial sequences of PCR products or of clones representative of each RFLP pattern obtained were determined by Qiagen Genomics Sequencing Services, using the internal primer Euk 528f (5′-GCG GTA ATT CCA GCT CCA A-3′; Elwood et al. 1985); (3).

I need to check that.

[vaulot]

But if I remember in the Moon paper, she was using primers coming from an obscure Korean paper (but she did not cite it..) - She was a post doc with me...

[frederic-mahe]

ok, it seems to be a dead-end. I could not find an older reference.