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primer3-org / primer3

Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).
GNU General Public License v2.0
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feature request: ability to specify left and right primer overhangs #14

Closed scottbecker closed 2 years ago

scottbecker commented 5 years ago

Some pcr experiments required adding overhangs to primers, e.g. restriction sites or homology arms. It would be nice to be able to tell primer3 about left and right primer overhangs that should be taken into account when doing hairpin and self-dimer scoring for the final primer.

bioinfo-ut commented 5 years ago

Good idea. Thanks for the suggestion.

untergasser commented 2 years ago

The overlap positions were added in https://github.com/primer3-org/primer3/commit/cbf451d1981adc1b9e35211f6fce45396c6e652a

It will be part of the next release.

Best, Andreas