Open vineetbansal opened 1 year ago
The actual sequence found in the fna
using grep
is AAGACTGTGCGCTAATCTCTTAG
(i.e. with the alt-pam), indicating that the match
reported in both the csv/sam cases is incorrect (the NGG
was automatically added). All such detected cases of specificity > 1 seem to be with matches that have the NAG
PAM.
When using the following kmer file:
with
guidescan enumerate
(versionv2.1.6
) againsthg38_noalt.index
(mismatches 3
andalt-pam NAG
), we get the following sam line:or the following csv lines (
succinct
mode):There's clearly something wrong here since specificity is reported > 1.