I have been trying to use the guidescan command line interface to basically run the gRNA Design tool from the website but I am a bit confused on the protocol.
As a test run I gave the following coordinates to the web interface (using hg38);
chr1:6526905-6528918
chr1:6529247-6531892
and was able to get the attached excel file as output.
But I don't know how to get the same output using the command line tool.
So far I tried the generate_kmers.py and then used that output as input for the guidescan enumerate subcommand but the output looks like below
Hi guys
I have been trying to use the guidescan command line interface to basically run the gRNA Design tool from the website but I am a bit confused on the protocol.
As a test run I gave the following coordinates to the web interface (using hg38);
and was able to get the attached excel file as output.
But I don't know how to get the same output using the command line tool.
So far I tried the
generate_kmers.py
and then used that output as input for the guidescan enumerate subcommand but the output looks like belowI would appreciate
results.xlsx