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pritykinlab / guidescanpy

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cpf1 sequence correction #66

Closed 1rzhu closed 1 year ago

1rzhu commented 1 year ago

guidescan enumerate has a --start flag, too. We didn't add this to the snakemake rule before, so the bam files appended the pam to the end of the sequences as default.

For example, the correct sequence of a target should be TTTNGGCCTAATACTAAGCCTAAG, but the bam files generated from snakemake write it as GGCCTAATACTAAGCCTAAGTTTN.

This PR made a correction for this.

1rzhu commented 1 year ago

A reminder: We need to take care of the 0/1-indexed stuff before all new bam files are put into use.