qPCR4vir / ThDySec

Modeling of primers/probes hybridization on to a set of target viral sequences.

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qPCR for Akabane and Tahyna (Peribunyaviridae - family, Orthobunyavirus - genus) #10

Open qPCR4vir opened 5 years ago

qPCR4vir commented 5 years ago

Bunyavirales - order (Phylum:Negarnaviricota; Subphylum:Polyploviricotina; Class:Ellioviricetes)

Peribunyaviridae - family

Orthobunyavirus - genus

*** Akabane orthobunyavirus - specie

[x] seq. All GB ~429. NC_009895. Akabane virus segment M. 79 seq. Bis 86%, next 76%. Segment L only 10 near complete 12 000nt. 49 seq. Bis 89%. segment S. 100 seq. Bis 94%.
[x] primers Literatur
[ ] oligos
[x] primers, PCR
[ ] exp Akabane virus, (taxid:70566) (JA GAR 39).

California encephalitis orthobunyavirus - specie

California encephalitis virus + ...
Inkoo virus La Crosse virus *** Tahyna virus (LEIV 12812)

[ ] seq
[ ] primers Literatur
[ ] oligos
[x] primers, PCR
[x] exp

Bunyamwera orthobunyavirus - specie

Batai virus Bunyamwera virus Ngari virus

[ ] seq
[ ] primers Literatur
[ ] oligos
[x] primers, PCR
[x] exp

*** Nairoviridae - family. see #14

*** Phenuiviridae - family. see #13

Hantaviridae - family

Orthohantavirus - genus

specie: Puumala orthohantavirus (Sotkamo)

[ ] seq
[ ] primers Literatur
[ ] oligos
[ ] primers, PCR
[x] exp