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qasimyu / simuscop

a novel next-generation sequencing simulator using position and genomic contexts based error profiles
GNU General Public License v3.0
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General questions #18

Open gibberwocky opened 6 months ago

gibberwocky commented 6 months ago

Hi

A couple of general questions.

  1. Could a more robust sequencing profile be generated if an average was generated from several BAM files? So for instance, if sequencing profiles were generated from several different BAM+VCF files using seqToProfile, can some or all of the values from the resulting model profiles be averaged to reach a consensus profile?
  2. The duplication rate I'm getting from simulated reads is very low (0.6%). Is there a way to manipulate this during simulation to better reflect what I observe in my actual sequencing data?

Best

D