Closed dixi06 closed 1 year ago
I am trying to import dada2R files (rep seq) to QIIME2 for taxonomic classification. I need help with the steps mentioned below.
I tried to extract sequences using dada2:: getSequences(seqtab.nochim) and then saving the file with writeFasta(seqtab.nochime, "rep-seq.fasta")
When I tried to import it in QIIME2 it gives me an error like this There was a problem importing rep-seq.fasta:
rep-seq.fasta is not a(n) DNAFASTAFormat file:
Description on line 1 is missing an ID.
I tried other ways to convert the data frame into fasta as well but it is not recognizing the file.
Hi @dixi06, sorry for missing this, could you repost this to the QIIME 2 forum you'll receive better support there :)
I am trying to import dada2R files (rep seq) to QIIME2 for taxonomic classification. I need help with the steps mentioned below.
I tried to extract sequences using dada2:: getSequences(seqtab.nochim) and then saving the file with writeFasta(seqtab.nochime, "rep-seq.fasta")
When I tried to import it in QIIME2 it gives me an error like this There was a problem importing rep-seq.fasta:
rep-seq.fasta is not a(n) DNAFASTAFormat file:
Description on line 1 is missing an ID.
I tried other ways to convert the data frame into fasta as well but it is not recognizing the file.