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qiime2 / q2-dada2

QIIME 2 plugin wrapping DADA2
BSD 3-Clause "New" or "Revised" License
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Importing DADA2 R rep. sequences into QIIME2 #159

Closed dixi06 closed 1 year ago

dixi06 commented 1 year ago

I am trying to import dada2R files (rep seq) to QIIME2 for taxonomic classification. I need help with the steps mentioned below.

I tried to extract sequences using dada2:: getSequences(seqtab.nochim) and then saving the file with writeFasta(seqtab.nochime, "rep-seq.fasta")

When I tried to import it in QIIME2 it gives me an error like this There was a problem importing rep-seq.fasta:

rep-seq.fasta is not a(n) DNAFASTAFormat file:

Description on line 1 is missing an ID.

I tried other ways to convert the data frame into fasta as well but it is not recognizing the file.

ebolyen commented 1 year ago

Hi @dixi06, sorry for missing this, could you repost this to the QIIME 2 forum you'll receive better support there :)