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qiime2 / q2-demux

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Golay correction not working as expected #161

Closed colinbrislawn closed 4 months ago

colinbrislawn commented 6 months ago

Here is what forum user dfground found in forum x-ref.

barcode-uncorrected 1 (GTA TTT ACG ATC) and barcode-uncorrected 2 (GTA TTA ACG ATC) have been corrected to barcode unused index (GTA TTG ACG GTC) by golay-error-correction. 

barcode-uncorrected 1 GTATTTACGATC
unused index 1        GTATTGACGGTC
                      ***** ***.**
barcode-uncorrected 2 GTATTAACGATC
unused index 2        GTATTGACGGTC
                      *****.***.**

Both barcode-uncorrected 1 and barcode-uncorrected 2 were thought to be caused by the insertion of a single base into used index 1 (GTA TTA CGA TCC).

barcode-uncorrected 1 GTATTTACGATC-
used index 1          GTATT-ACGATCC
                      ***** ******
barcode-uncorrected 2 GTATTAACGATC-
used index 1          GTATTA-CGATCC
                      ****** *****