qiime2 / q2-demux

BSD 3-Clause "New" or "Revised" License
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support for paired end, variable length demultiplexing #39

Open gregcaporaso opened 7 years ago

gregcaporaso commented 7 years ago

Comments This could be a new method in this plugin.

References A user requested this support on the forum here.

jairideout commented 6 years ago

Came up again on the forum.

thermokarst commented 6 years ago

This recently came up on the forum.

lstanish commented 5 years ago

Any updates on this functionality? All of the recent NEON data are dual-indexed.

thermokarst commented 5 years ago

I think this issue can be closed, because I believe you can accomplish this using q2-cutadapt (but please correct me if I am wrong).

lstanish commented 5 years ago

I have the same issue as reported here, separate index files (I1 and I2) need to be merged with sequence files (R1 and R2) before demultiplexing. Looks like q2-cutadapt only works when the barcodes are already attached to the sequences?