quadbio / scMultiome_analysis_vignette

Tutorial of single-cell RNA-ATAC multiomic sequencing data analysis
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RegionStats and LinkPeaks for rhesus datasets #6

Open sylestiel opened 6 months ago

sylestiel commented 6 months ago

Hi @zhisonghe,

I was trying to use your Multiome vignette to analyze rhesus datasets using the Macaca_mulatta.Mmul_10.109.gtf file from the ensembl database. However, I'm having issues with the RegionsStats() and LinkPeaks() steps.

If the seqlevelsStyle(seurat) is 'NCBI' how do I specify the appropriate genome to run the above functions? Alternatively, can I format the BSgenome.Mmulatta.UCSC.rheMac10 genome to use that instead?

Hope you can help with some suggestions!

Thanks!