Whet the annotated VCF has Mastermind URL in CSQ tags, vcf2db.py throw the following error:
Traceback (most recent call last):
File "/opt/vcf2db/vcf2db.py", line 923, in
impacts_extras=a.impacts_field, aok=a.a_ok)
File "/opt/vcf2db/vcf2db.py", line 233, in init
self.load()
File "/opt/vcf2db/vcf2db.py", line 318, in load
i = self._load(self.cache, create=True, start=1)
File "/opt/vcf2db/vcf2db.py", line 311, in _load
self.insert(variants, expanded, keys, i, create=create)
File "/opt/vcf2db/vcf2db.py", line 340, in insert
v in variants)
File "/opt/vcf2db/vcf2db.py", line 809, in gene_info
impacts.extend(cls(e, impacts_headers[k]) for e in dk)
File "/opt/vcf2db/vcf2db.py", line 809, in
impacts.extend(cls(e, impacts_headers[k]) for e in dk)
File "/opt/conda/lib/python2.7/site-packages/geneimpacts/effect.py", line 530, in init
assert not "=" in effect_string
AssertionError
Whet the annotated VCF has Mastermind URL in CSQ tags, vcf2db.py throw the following error:
Traceback (most recent call last): File "/opt/vcf2db/vcf2db.py", line 923, in
impacts_extras=a.impacts_field, aok=a.a_ok)
File "/opt/vcf2db/vcf2db.py", line 233, in init
self.load()
File "/opt/vcf2db/vcf2db.py", line 318, in load
i = self._load(self.cache, create=True, start=1)
File "/opt/vcf2db/vcf2db.py", line 311, in _load
self.insert(variants, expanded, keys, i, create=create)
File "/opt/vcf2db/vcf2db.py", line 340, in insert
v in variants)
File "/opt/vcf2db/vcf2db.py", line 809, in gene_info
impacts.extend(cls(e, impacts_headers[k]) for e in dk)
File "/opt/vcf2db/vcf2db.py", line 809, in
impacts.extend(cls(e, impacts_headers[k]) for e in dk)
File "/opt/conda/lib/python2.7/site-packages/geneimpacts/effect.py", line 530, in init
assert not "=" in effect_string
AssertionError
Script submitted is as below:
vcf2db.py \ ${VCF_IN} \ ${PED} \ ${DB_OUT} \ --expand gt_types \ --expand gt_ref_depths \ --expand gt_alt_depths
Input files attached. hg38validation_quartet.30t.vcf.gz
hg38validation_quartet.vcf.ped.txt