Closed yeswzc closed 4 years ago
I found the reason:
After findDAR
, there is no significant DARs identified, so the vector idy
is empty.
It is weird that no significant DARs were found. I will keep on look into this issue.
Hi,
It turns out when using findDAR on my data. cluster.neg.method = "knn"
can not find significant DARs.
However, when I try cluster.neg.method="random"
or cluster.neg.method="other"
(I "hacked" into the code :) ), the output plot is weird. The cluster with the highest z-scored read count (I guess) is not the cluster as we expected. For example, when I sought for DARs in cluster 10, the output showed cluster 9 had the highest (yellow points in figure) but cluster 10 were totally dark, when I sought for DARs in cluster 11, the output showed cluster 2 had the highest.
Except for tested test.method = "exactTest"
I also tested "LRT"
, similar results.
Maybe my data is not applicable?
Hi, I met an error with my own data in Step 14 Identify differentially accessible regions (10X Adult Mouse Brain). Do you know how to solve this?
Thank you!