Tannic acid concentration pending confirmation

[ramiromagno]

Tannic acid concentrations

In the original paper, the tannic acid concentrations are described as: 5, 1, 0.2, 0.04 and 0.08 ng per 25 uL of PCR volume. This gives 0.2 ug / mL for the most concentrated reactions, which sounds maybe a bit too much on the lower end.

Other papers

Kontanis & Reed (2006)

Concentrations in Kontanis & Reed (2006) seem to be seven-fold higher:

Tannic acid powder (Sigma, St. Louis, MO) was serially diluted (0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4 ng/mL) in water (Sigma) to act as the inhibitory agent.

Sisti et al. (2010)

Concentrations in Sisti et al. (2010) seem to be thousand-fold higher:

Tannic acid oxidizes to form quinones which covalently bind to Taq DNA polymerase inhibiting its activity [31]. Real-time amplification plots from 3.5 × 104 DNA molecules in the presence of increasing concentrations (0-0.1 mg per mL) of tannic acids were obtained.

[ramiromagno]

Pending feedback from the original author.