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ratschlab / spladder

Tool for the detection and quantification of alternative splicing events from RNA-Seq data.
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Interpretation of the differential splicing testing results #187

Open sneha100895 opened 1 year ago

sneha100895 commented 1 year ago

Hi,

I have successfully run the differential splicing analysis using spladder, but I need some help with interpreting certain columns in the output file : test_resultsC{C}{ET}.tsv

  1. What does the binary values in the "alt_usage" column mean? I am assuming that a value of 0 indicates absence and 1 indicates the presence of the exon in the transcript. Is it correct?

  2. How exactly is the mean_event_count (mean support for tested splice path) calculated?

Thank you!