Closed wkc1986 closed 4 years ago
Hello,
Thanks for giving the version number, I think that's the key to this mystery. Excuse me if you already know this, but release versions of Bioconductor packages are even (e.g. current release for annotatr is 1.14.0) and devel versions are odd (e.g. 1.15.0). Sometimes when there is a mixture of release and devel packages, things can get a little weird.
When I spin up the rcavalcante/r4.0_bioc3.11 docker image, I don't get the warnings you did for which
, and the build_annotations()
function works with the command you give.
I wonder, can you do BiocManager::valid()
and give the result here?
Thanks.
Thanks for the response. Yes, I had to install the developer version of another package. Here's output of valid()
:
> BiocManager::valid()
* sessionInfo()
R version 4.0.0 (2020-04-24)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Mojave 10.14.6
Matrix products: default
BLAS: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRblas.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] org.Mm.eg.db_3.11.1
[2] TxDb.Mmusculus.UCSC.mm10.knownGene_3.10.0
[3] GenomicFeatures_1.41.0
[4] AnnotationDbi_1.51.0
[5] Biobase_2.49.0
[6] GenomicRanges_1.41.5
[7] GenomeInfoDb_1.25.0
[8] IRanges_2.23.9
[9] S4Vectors_0.27.12
[10] BiocGenerics_0.35.2
[11] annotatr_1.15.0
loaded via a namespace (and not attached):
[1] httr_1.4.1 regioneR_1.21.0
[3] bit64_0.9-7 AnnotationHub_2.21.0
[5] shiny_1.4.0.2 assertthat_0.2.1
[7] interactiveDisplayBase_1.27.0 askpass_1.1
[9] BiocManager_1.30.10 BiocFileCache_1.13.0
[11] blob_1.2.1 BSgenome_1.57.0
[13] GenomeInfoDbData_1.2.3 Rsamtools_2.5.0
[15] yaml_2.2.1 progress_1.2.2
[17] BiocVersion_3.12.0 pillar_1.4.4
[19] RSQLite_2.2.0 lattice_0.20-41
[21] glue_1.4.1 digest_0.6.25
[23] promises_1.1.0 XVector_0.29.2
[25] colorspace_1.4-1 plyr_1.8.6
[27] htmltools_0.4.0 httpuv_1.5.2
[29] Matrix_1.2-18 XML_3.99-0.3
[31] pkgconfig_2.0.3 biomaRt_2.45.0
[33] zlibbioc_1.35.0 purrr_0.3.4
[35] xtable_1.8-4 scales_1.1.1
[37] later_1.0.0 BiocParallel_1.23.0
[39] tibble_3.0.1 openssl_1.4.1
[41] ggplot2_3.3.0 ellipsis_0.3.1
[43] SummarizedExperiment_1.19.5 magrittr_1.5
[45] crayon_1.3.4 mime_0.9
[47] memoise_1.1.0 tools_4.0.0
[49] prettyunits_1.1.1 hms_0.5.3
[51] lifecycle_0.2.0 matrixStats_0.56.0
[53] stringr_1.4.0 munsell_0.5.0
[55] DelayedArray_0.15.1 Biostrings_2.57.2
[57] compiler_4.0.0 rlang_0.4.6
[59] grid_4.0.0 RCurl_1.98-1.2
[61] rappdirs_0.3.1 bitops_1.0-6
[63] gtable_0.3.0 DBI_1.1.0
[65] curl_4.3 reshape2_1.4.4
[67] R6_2.4.1 GenomicAlignments_1.25.0
[69] dplyr_0.8.5 rtracklayer_1.49.1
[71] fastmap_1.0.1 bit_1.1-15.2
[73] readr_1.3.1 stringi_1.4.6
[75] Rcpp_1.0.4.6 vctrs_0.3.0
[77] dbplyr_1.4.3 tidyselect_1.1.0
Bioconductor version '3.12'
* 66 packages out-of-date
* 0 packages too new
create a valid installation with
BiocManager::install(c(
"AnnotationDbi", "AnnotationHub", "annotatr", "backports", "BiocGenerics",
"BiocParallel", "biomaRt", "bit64", "broom", "BSgenome", "bsseq", "dbplyr",
"DelayedArray", "DelayedMatrixStats", "dplyr", "DT", "fs", "GenomeInfoDb",
"GenomicAlignments", "GenomicFeatures", "ggforce", "ggplot2", "ggraph",
"Gviz", "haven", "HDF5Array", "htmlTable", "htmltools", "httpuv",
"interactiveDisplayBase", "IRanges", "isoband", "jsonlite", "knitr",
"later", "limma", "lubridate", "openssl", "org.Mm.eg.db", "pillar",
"pkgbuild", "pkgload", "processx", "promises", "Rcpp", "RcppArmadillo",
"regioneR", "rhdf5", "Rhdf5lib", "Rhtslib", "rlang", "rmarkdown",
"roxygen2", "Rsamtools", "rtracklayer", "rversions", "shiny",
"SummarizedExperiment", "tibble", "tidyr", "tinytex", "VariantAnnotation",
"vctrs", "xfun", "XML", "XVector"
), update = TRUE, ask = FALSE)
more details: BiocManager::valid()$too_new, BiocManager::valid()$out_of_date
Warning message:
66 packages out-of-date; 0 packages too new
Thanks for that, so it seems like you're consistently using devel
, which rules out the mixture issue.
It's a little strange to me that the devel builds are fine on Bioconductor, but it doesn't work for you. It would be helpful to know which package is causing the which
problem... let me update my devel Docker image and see if the problem is replicated there.
I just updated all the packages in my devel Docker image rcavalcante/rdevel_bioc3.12, and couldn't replicate the problem either. Hmm.
> BiocManager::valid()
[1] TRUE
> sessionInfo()
R version 4.0.0 (2020-04-24)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 18.04.4 LTS
Matrix products: default
BLAS/LAPACK: /usr/lib/x86_64-linux-gnu/libopenblasp-r0.2.20.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=C
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] org.Mm.eg.db_3.11.4
[2] TxDb.Mmusculus.UCSC.mm10.knownGene_3.10.0
[3] GenomicFeatures_1.41.2
[4] AnnotationDbi_1.51.1
[5] Biobase_2.49.0
[6] GenomicRanges_1.41.5
[7] GenomeInfoDb_1.25.8
[8] IRanges_2.23.10
[9] S4Vectors_0.27.12
[10] BiocGenerics_0.35.4
[11] annotatr_1.15.2
[12] BiocManager_1.30.10
loaded via a namespace (and not attached):
[1] httr_1.4.1 regioneR_1.21.1
[3] bit64_0.9-7.1 AnnotationHub_2.21.1
[5] shiny_1.5.0 assertthat_0.2.1
[7] interactiveDisplayBase_1.27.5 askpass_1.1
[9] BiocFileCache_1.13.0 blob_1.2.1
[11] BSgenome_1.57.4 GenomeInfoDbData_1.2.3
[13] Rsamtools_2.5.3 yaml_2.2.1
[15] progress_1.2.2 BiocVersion_3.12.0
[17] pillar_1.4.6 RSQLite_2.2.0
[19] lattice_0.20-41 glue_1.4.1
[21] digest_0.6.25 promises_1.1.1
[23] XVector_0.29.3 colorspace_1.4-1
[25] plyr_1.8.6 htmltools_0.5.0
[27] httpuv_1.5.4 Matrix_1.2-18
[29] XML_3.99-0.4 pkgconfig_2.0.3
[31] biomaRt_2.45.2 zlibbioc_1.35.0
[33] purrr_0.3.4 xtable_1.8-4
[35] scales_1.1.1 later_1.1.0.1
[37] BiocParallel_1.23.2 tibble_3.0.3
[39] openssl_1.4.2 generics_0.0.2
[41] ggplot2_3.3.2 ellipsis_0.3.1
[43] SummarizedExperiment_1.19.6 magrittr_1.5
[45] crayon_1.3.4 mime_0.9
[47] memoise_1.1.0 tools_4.0.0
[49] prettyunits_1.1.1 hms_0.5.3
[51] lifecycle_0.2.0 matrixStats_0.56.0
[53] stringr_1.4.0 munsell_0.5.0
[55] DelayedArray_0.15.7 Biostrings_2.57.2
[57] compiler_4.0.0 rlang_0.4.7
[59] grid_4.0.0 RCurl_1.98-1.2
[61] rappdirs_0.3.1 bitops_1.0-6
[63] gtable_0.3.0 DBI_1.1.0
[65] curl_4.3 reshape2_1.4.4
[67] R6_2.4.1 GenomicAlignments_1.25.3
[69] dplyr_1.0.0 rtracklayer_1.49.3
[71] fastmap_1.0.1 bit_1.1-15.2
[73] readr_1.3.1 stringi_1.4.6
[75] Rcpp_1.0.5 vctrs_0.3.2
[77] dbplyr_1.4.4 tidyselect_1.1.0
I wonder, could you try updating your packages and see if the problem persists?
After updating R to 4.0.2 and all packages except vctrs
(staying on latest binary version) it works. Not sure where the problem was but it seems to be gone. Thank you.
From a fresh start:
R 4.0.0, annotatr 1.15.0. I'd appreciate it if you could help, thanks!