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rcedgar / muscle

Multiple sequence and structure alignment with top benchmark scores scalable to thousands of sequences. Generates replicate alignments, enabling assessment of downstream analyses such as trees and predicted structures.
https://drive5.com/muscle
GNU General Public License v3.0
203 stars 22 forks source link

Windows version does not work myalloc64(4, 1.1e+09) failed #18

Closed yeyun1999 closed 2 years ago

yeyun1999 commented 2 years ago

muscle 5.0.1428_win64 34.1Gb RAM, 16 cores (C) Copyright 2004-2021 Robert C. Edgar. https://drive5.com

00:00 5.9Mb 0.037% Calc posteriors

D:\MyApp\bin\muscle.exe -align .\all.fasta -output all2.fasta Elapsed time 00:04 Max memory 5.9Mb

---Fatal error--- myalloc64(4, 1.1e+09) failed

rcedgar commented 2 years ago

what is the sequence length?

rcedgar commented 2 years ago

This error is probably caused by very long sequences in the input. In v5.1, muscle reports the mean and maximum sequence lengths when starting and issues a warning if long sequences are found.