Multiple sequence and structure alignment with top benchmark scores scalable to thousands of sequences. Generates replicate alignments, enabling assessment of downstream analyses such as trees and predicted structures.
Hej Robert!
I'm running muscle5 on a computing cluster and all seems to work super smooth, but I've run into a small issue when trying to calculate the column confidence.
Hej Robert! I'm running muscle5 on a computing cluster and all seems to work super smooth, but I've run into a small issue when trying to calculate the column confidence.
muscle -addconfseqs Gene_0107_diversified_ensemble.efa -output Gene_0107_diversified_ensemble_confseqs.efa
Below the output from the log file:
Invalid command line Unknown option addconfseqs
muscle 5.1.linux64 [] 131Gb RAM, 20 cores Built Mar 29 2022 15:27:13 (C) Copyright 2004-2021 Robert C. Edgar. https://drive5.com
Is this an issue at my end or yours? Cheers, Karin