Multiple sequence and structure alignment with top benchmark scores scalable to thousands of sequences. Generates replicate alignments, enabling assessment of downstream analyses such as trees and predicted structures.
This is very minor: Using the old syntax muscle -in input.fasta -out output.aln is not compatible anymore with version 5, but the error message does not point in the right direction. Instead of
Invalid command line
Unknown option in
It may be helpful to show something like
The option `--in` is no longer supported. Please use `--align` instead.
rcedgar
commented
1 year ago
This is very minor: Using the old syntax
muscle -in input.fasta -out output.alnis not compatible anymore with version 5, but the error message does not point in the right direction. Instead ofIt may be helpful to show something like